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KMID : 0380619840160030368
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Korean Journal of Food Science and Technology
1984 Volume.16 No. 3 p.368 ~ p.374
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Study on the Purification of 5 ´ - Ribonucleotide
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Abstract
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The purification of 5´-riboncleotide using ion exchange resins has been studied and the optimum conditions were determined. The amount used of Amberlite IR120 ion exchange resin in 2nd resin tower could be reduced up to 20% by pretreating the hydrolyzed RNA solution in 1st resin tower. The amount used of regenerant could be also reduced up to 20% by desalting the hydrolyzed RNA solution in the 1st tower, because the desalted solution eluted easily by the water in the 2nd tower. The crystal obtained in this experiment was the mixed-crystals of 5´-IMP.Na₂= and 5´-GMP.Na₂. The crystallization of the complexes formed from 5´-IMP.Na₂ and 5´-GMP.Na₂= gave the best result at pH 7.6. The yield of crystal complexes formed from 5´-IMP.Na₂= and S´-GMP.Na₂ was obtained higher in high MeOH concentration. However, in higher than 6096 MeOH concentration the products was amorphous. The higher content of MeOH for the crystallization of the product gave the smaller value of 5´-IMP.Na₂/5´-GMP.Na₂.
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